Journal: Scientific reports

Article Title: D-Cateslytin, a new antimicrobial peptide with therapeutic potential.

doi: 10.1038/s41598-017-15436-z

Figure Lengend Snippet: Figure 4. Stability of D-Ctl and L-Ctl towards proteases secreted by different bacterial strains. Supernatants from E. coli wild type (A and B), E. coli MDR (C and D), F. nucleatum (E and F), P. intermedia (G and H), P. micra (I and J), S. aureus methicillin sensitive (MSSA) (K), S. aureus methicillin resistant (MRSA) (L) were incubated with D-Ctl or L-Ctl, as indicated, for 24 hours. Peptide stability was then assessed by HPLC. Chromatograms 1 correspond to supernatant only, chromatograms 2 correspond to supernatant and peptide and chromatograms 3 corresponds to peptide only.

Article Snippet: We then investigated whether D-Ctl could potentiate the antibacterial effect of several antimicrobials of reference, specifically methicillin and vancomycin extensively prescribed to treat S. aureus infections, amoxicillin recommended in numerous Pathogen Gram Respiratory type MIC (peptide) Antibiotic of reference L-Ctl (μg/mL) D-Ctl (μg/mL) Name (μg/mL) Escherichia coli (ATCC 25922) − Facultative anaerobe 75 8.0 Ampicillin 7.0 Kanamycin 21 Escherichia coli (MDR) (K-12) − Facultative anaerobe 150 8.4 Cefotaxime 0.1 Fusobacterium nucleatum (ATCC 49256) − Obligate anaerobe 125 22 Amoxicillin 0.6 Prevotella intermedia (ATCC 49046) − Obligate anaerobe 149 10 Amoxicillin 0.5 Parvimonas micra (ATCC 33270) + Obligate anaerobe 120 23 Amoxicillin 0.5 Staphylococcus aureus (MSSA) (ATCC 25923) + Facultative anaerobe 40* 24 Methicillin 1.2 Staphylococcus aureus (MRSA) (S1) + Facultative anaerobe 37* 18 Vancomycin 0.8 Table 1.

Techniques: Incubation

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Increased levels of specific microbial markers are detected in faeces of CRC patients. Scatter plots are used to illustrate the relative levels of ( A ) P. micra ( Pm ), ( B ) F. nucleatum ( Fn ), and ( C ) clbA + bacteria ( clbA ) in faeces of control patients, and patients diagnosed with dysplasia or CRC from the FECSU and U-CAN cohorts. Horizontal lines indicate mean relative expression calculated by the 2 -ΔCq method with the total microbial 16S rRNA gene DNA as reference.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Control, Expressing

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: ROC curves displaying the specificity and the sensitivity for P. micra ( Pm ), F. nucleatum ( Fn ), and clbA + bacteria ( clbA ) to detect CRC. ROC-curves were calculated using the levels for the specific marker as indicated and cancer/no cancer. The levels of a specific marker in each sample was given as a relative quantification calculated by the 2 -ΔCt method with the total microbial 16S rRNA gene DNA as reference.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Marker, Quantitative Proteomics

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Performance of single faecal microbial markers or combinations of markers in CRC detection. Sensitivity and specificity for CRC detection is displayed for a test of ( A ) P. micra ( Pm ), F. nucleatum ( Fn ), or clbA + bacteria ( clbA ), as well as combined tests using several microbial markers for the FECSU and U-CAN cohort, and ( B ) for combined tests using microbial markers and immunochemical F-Hb (Hb) for the FECSU cohort. For test 1, a positive test result was given to samples with at least one positive marker. For test 2, a positive test result was given to samples with at least two positive markers.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Marker

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: The distribution of specific microbial markers in faeces of CRC patients. Circle diagrams are used to illustrate the abundance of P. micra ( Pm ), F. nucleatum ( Fn ) and clbA + bacteria ( clbA ) in faecal samples with all three markers evaluated of control patients, and patients diagnosed with dysplasia or CRC from the FECSU and U-CAN cohorts.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Control

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Microbial alterations in faeces of study patients from the FECSU cohort.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Control, Bacteria